Please find the CMA 8 Elite probe manuals here. 

Item Numbers:

• CMA8012201 CMA 8 Elite 1mm membrane length
• CMA8012202 CMA 8 Elite 2mm membrane length
• CMA8012301 CMA 8 High Cut Off 1mm membrane length
• CMA8012302 CMA 8 High Cut Off 2mm membrane length

CMA 8 Elite Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Attach the Microdialysis probe to a CMA 7 & 8 Probe/Guide Clip on the CMA 130 In Vitro Stand. Remove the protection tube carefully. Put the probe membrane into a vial filled with perfusion fluid.
4. Connect a Tubing Adapter to the blue inlet tubing of the Microdialysis probe and connect it to the syringe cannula by sliding the Tubing Adapter over the cannula. To facilitate the handling of Tubing Adapters, they should be soaked in Ethanol for a minimum of 10 minutes.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min. The Tubing Adapter must be dry before flushing.
6. Flush the probe with the 10-15µL/min in the Perfusion Fluid for 4-5min to wash out air. Check for air bubbles inside the membrane with a stereomicroscope. The membrane is light blue when wetted, air bubbles occur as whiter spots. When flushing the membrane may appear to be "sweating" which is due to the ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times. 
9. After the experiment put the microdialysis probe in a vial filled with deionized water. Perfuse with deinonized water to prevent salt crystal formation. The probe can be stored in deionized water.

CMA 8 High Cut Off 

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Connect a Tubing Adapter to the blue inlet tubing of the Microdialysis probe and connect it to the syringe cannula by sliding the Tubing Adapter over the cannula. Don't add longer outlet tubing than necessary to avoid ultrafiltration. No longer than 500mm inlet tubing = blue, outlet tubing = transparent. To facilitate the handling of Tubing adatpers, they should be soaked in Ethanol for a minimum 10 minutes.
4. Attach the Microdialysis probe to a CMA 7 & 8 Probe/Guide Clip on the CMA 130 in vitro Stand. Remove the protection tube carefully. Put the probe membrane into a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 minutes. The Tubing Adapter must be dry before flushing.
6. Flush the probe with perfusion fluid at 8-10µL/min for 3-4min to wash out air. When flushing, the membrane may appear to be "sweating" which is due to ultrafiltration of fluid through the membrane. Knock on the shaft of the clip to help flush out the air. Lift up the clip with the probe from the vial and check for air bubbles inside the membrane with a microscope. Air bubbles occur as white spots.
7. Set the pump to the required perfusion flow, usually 1-2µL/min and check for leaks. Keep pump, probe and tubing at the same level on the bench to prevent ultrafiltration. If the membrane still sweats it might  still be air inside the probe. Repeat step 6. It might be helpful to change flow direction in the probe by connecting the inlet tubing to the outlet on the probe for a minute. Use Dextran MW 500kDa 3% to prevent ultrafiltration. 
8. When the membrane is not sweating the system with the probe is ready to use.
9. During the experiment remember to check the fluid volume in the vials to be as calculated. If a higher flow rate than 1-2µL/min is required it is recommended to use a push-pull system to avoid ultrafiltration.
10. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
11. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation. The probe can be stored in deionized water.