Please find the CMA 7 Microdialysis Probe manuals.

• CMAP000082 CMA 7 6 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMAP000083 CMA 7 6 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8010771 CMA 7 6 kDa Metal Free Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8010772 CMA 7 6 kDa Metal Free Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012411 CMA 7 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012412 CMA 7 55 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012421 CMA 7 500 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012422 CMA 7 500 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012423 CMA 7 2 MDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012424 CMA 7 2 MDa Microdialysis Probe, 2 mm membrane length, pkg. of 3

CMA 7 Microdialysis Probe 6kDa

1. Fill a microsyringe with Perfusion Fluid and mount it in the CMA Microinjection Pump. The Perfusion Fluid must be clean, at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 7 & 8 Probe/Guide Clip. Put the probe in a vial filled with Perfusion Fluid on the CMA 130 in vitro Stand or in the in vitro holder on the CMA 402 Pump. 
5. Connect a Tubing Adapter and required length of FEP tubing to the blue inlet tubing of the microdialysis probe and then connect it to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing.
6. Flush the probe with 10-15µL/min in the Perfusion Fluid for 4-5min to wash out air. While flushing, "tap" with a tool on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tapping" must be repeated. When flushing the membrane it may appear to be "sweating" which is due to ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow, usually 1-5µL/min and check for leaks.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes  a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation.

CMA 7 55kDa Microdialysis Probe

1. Fill a microsyringe with Perfusion Fluid and mount it in the CMA Microinjection Pump. The Perfusion Fluid must be clean, at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 7 & 8 Probe/Guide Clip. Put the probe in a vial filled with Perfusion Fluid on the CMA 130 in vitro Stand or in the in vitro holder on the CMA 402 Pump. 
5. Connect a Tubing Adapter and required length of FEP tubing to the blue inlet tubing of the microdialysis probe and then connect it to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing.
6. Flush the probe with 10-15µL/min in the Perfusion Fluid for 4-5min to wash out air. While flushing, "tap" with a tool on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tapping" must be repeated. When flushing the membrane it may appear to be "sweating" which is due to ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow, usually 1-5µL/min and check for leaks.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes  a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation.

CMA 7 500kDa & 2MDa Microdialysis Probes

Note: the 500kDa and 2Mda Cut Off membrane has to be used in a Push-Pull system to avoid loss of fluid out to the surrounding tissue (ultra-filtration). Here the use of a CMA syringe pump and REGLO ICC Pump is recommended. 

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Load the peristaltic pump with the "FEP Tubing Connector Peristaltic Kit." Adjust the lengths of the FEP tubing if needed for a lower dead volume; recalculate the outlet volume if needed (1.2µL/100mm length)
4. Place the inlet end of the "FEP Tubing Connector Peristaltic Kit" in a beaker with Perfusion Fluid and flush the pump to fill all tubing with perfusion fluid. Make sure there is no air bubbles in the complete tubing set.
5. Set the pump to the required perfusion flow, usually 1-5µL/min.
6. Prepare a desired length of FEP tubing with a Tubing Adapter on both ends and connect the tubing to the inlet of the probe. Remove the protection tube from the probe carefully. Blue = inlet, Transparent = Outlet. Tubing Adapters and FEP tubing should be used for all connections. To facilitate the handling of Tubing Adapters, they should be soaked in Ethanol 70% for minimum 10 minutes.
7. Mount the probe in a Probe/Guide Clip on the CMA 130 In Vitro Stand. Put the probe membrane into a vial filled with perfusion fluid. Connect the inlet tubing of the probe to the syringe cannula by sliding the Tubing Adapter over the cannula.
8. Flush the probe with perfusion fluid at 8-10µL/min for 4-5 min to wash out air. Knock on the shaft of the clip to help the air to flush away. At this process the Ultra High Cut Off membrane will look as leaking but this is due to ultra-filtration of fluid through the membrane.
9. Set the pump to the required perfusion flow, usually 1-5µL/min. Make sure both pumps have the same flow rate.
10. Connect the inlet "FEP Tubing Connector Peristaltic Kit" at the peristaltic pump to the outlet of the probe
11. Lift up the probe from the vial and check that the membrane doesn't ultrafiltrate or dry out; it should look filled out but not sweating. Lower the probe into the vial and control the outlet volume for the complete system. 
12. The system with the probe is now ready for use.
13. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.