Ideal for discrete areas in the CNA due to its greater spatial resolution and reduced size. Please use this article for the CMA 11 probes.

• CMA8309581 CMA 11 6 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8309582 CMA 11 6 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8309583 CMA 11 6 kDa Microdialysis Probe, 3 mm membrane length, pkg. of 3
• CMA8309584 CMA 11 6 kDa Microdialysis Probe, 4 mm membrane length, pkg. of 3
• CMA8011081 CMA 11 6 kDa Metal Free Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8011082 CMA 11 6 kDa Metal Free Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8011083 CMA 11 6 kDa Metal Free Microdialysis Probe, 3 mm membrane length, pkg. of 3
• CMA8011084 CMA 11 6 kDa Metal Free Microdialysis Probe, 4 mm membrane length, pkg. of 3
• CMA8012511 CMA 11 55 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012512 CMA 11 55 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012513 CMA 11 55 kDa Microdialysis Probe, 3 mm membrane length, pkg. of 3
• CMA8012514 CMA 11 55 kDa Microdialysis Probe, 4 mm membrane length, pkg. of 3
• CMA8012521 CMA 11 500 kDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012522 CMA 11 500 kDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012523 CMA 11 500 kDa Microdialysis Probe, 3 mm membrane length, pkg. of 3
• CMA8012524 CMA 11 500 kDa Microdialysis Probe, 4 mm membrane length, pkg. of 3
• CMA8012525 CMA 11 2 MDa Microdialysis Probe, 1 mm membrane length, pkg. of 3
• CMA8012526 CMA 11 2 MDa Microdialysis Probe, 2 mm membrane length, pkg. of 3
• CMA8012527 CMA 11 2 MDa Microdialysis Probe, 3 mm membrane length, pkg. of 3
• CMA8012528 CMA 11 2 MDa Microdialysis Probe, 4 mm membrane length, pkg. of 3

CMA 11 6kDa Cuprophane Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 11 & 12 Probe Clip on the CMA 130 In Vitro Stand or the In Vitro holder on the CMA 402 Pump. Put the microdialysis probe in a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing. 
6. Flush the probe with 10-15µL/min in the Perfusion Fluid for 4-5min to wash out air. While flushing, "ta" with a scissor on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tapping" must be repeated. When flushing the membrane it may appear to be "sweating" which is due to the ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation. The probe can be stored in deionized water.
10. For further setup instructions, see CMA 120 System for Freely Moving Animals, User's Manual. 

CMA 11 55kDa Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 11 & 12 Probe Clip on the CMA 130 In Vitro Stand or the In Vitro holder on the CMA 402 Pump. Put the microdialysis probe in a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing. 
6. Flush the probe with 8-10µL/min in the Perfusion Fluid for 4-5min to wash out air. While flushing, "tap" with a scissor on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tapping" must be repeated. The membrane is light blue when wetted, air bubbles occur as whiter spots. When flushing the membrane it may appear to be "sweating" which is due to the ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation. The probe can be stored in deionized water.
10. For further setup instructions, see CMA 120 System for Freely Moving Animals, User's Manual. 

CMA 11 500kDa & 2MDa Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Load the peristaltic pump with the "FEP Tubing Connector Peristaltic Kit." Adjust the length of the FEP tubing if needed for a lower dead volume; recalculate the outlet volume if needed (1.2µL/100mm length).
4. Place the inlet end of the "FEP Tubing Connector Peristaltic Kit" in a beaker with Perfusion Fluid and flush the pump to fill all tubing with perfusion fluid. Make sure there are no air bubbles in the complete tubing set.
5. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. The microdialysis probe is now ready for use.
6. Prepare a desired length of tubing with a Tubing Adapter on both ends and connect the tubing to the inlet of the probe. Remove the protection tube from the probe carefully. Short cannula = inlet, long cannula = outlet. Tubing Adapters and FEP tubing should be used for all connections. To facilitate the handling of Tubing Adapters, they should be soaked in Ethanol 70% for a minimum of 10 minutes.
7. Mount the probe in a Probe/Guide Clip on the CMA 130 In Vitro Stand. Put the probe membrane into a vial filled with perfusion fluid. Connect the inlet tubing of the probe to the syringe cannula by sliding the Tubing Adapter over the cannula.
8. Flush the probe with perfusion fluid at 8-10µL/min for 4-5 min to wash out air. Knock on the shaft of the clip to help the air to flush away. During this process, the Ultra High Cut Off membrane will look like it is leaking but this is due to the ultra-filtration of fluid through the membrane.
9. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. Make sure both pumps have the same flow rate.
10. Connect the inlet "FEP Tubing Connector Peristaltic Kit" at the peristaltic pump to the outlet of the probe.
11. Lift the probe from the vial and check that the membrane doesn't ultrafiltrate or dry out; it should looked filled out but not sweating. Lower the probe into the vial and control the outlet volume for the complete system.
12. The system with the probe is now ready for use.
13. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.