Ideal for stereotaxic work in the CNS of anesthetized or conscious animals.

• CMA8010431 CMA 12 Elite Probe, 1 mm membrane length, pkg. of 3
• CMA8010432 CMA 12 Elite Probe, 2 mm membrane length, pkg. of 3
• CMA8010433 CMA 12 Elite Probe, 3 mm membrane length, pkg. of 3
• CMA8010434 CMA 12 Elite Probe, 4 mm membrane length, pkg. of 3
• CMA8309661 CMA 12 HighCO Probe, 1 mm membrane length, pkg. of 3
• CMA8309662 CMA 12 HighCO Probe, 2 mm membrane length, pkg. of 3
• CMA8309663 CMA 12 HighCO Probe, 3 mm membrane length, pkg. of 3
• CMA8309664 CMA 12 HighCO Probe, 4 mm membrane length, pkg. of 3
• CMA8011201 CMA 12 Elite Metal Free Probe, 1 mm membrane length, pkg. of 3
• CMA8011202 CMA 12 Elite Metal Free Probe, 2 mm membrane length, pkg. of 3
• CMA8011203 CMA 12 Elite Metal Free Probe, 3 mm membrane length, pkg. of 3
• CMA8011204 CMA 12 Elite Metal Free Probe, 4 mm membrane length, pkg. of 3
• CMA8011221 CMA 12 HighCO Metal Free Probe, 1 mm membrane length, pkg. of 3
• CMA8011222 CMA 12 HighCO Metal Free Probe, 2 mm membrane length, pkg. of 3
• CMA8011223 CMA 12 HighCO Metal Free Probe, 3 mm membrane length, pkg. of 3
• CMA8011224 CMA 12 HighCO Metal Free Probe, 4 mm membrane length, pkg. of 3

CMA 12 Elite Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 11 & 12 Probe Clip on the CMA 130 In Vitro Stand or the In Vitro holder on the CMA 402 Pump. Put the microdialysis probe in a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing. 
6. Flush the probe with 10-15µL/min in the Perfusion Fluid for 4-5min to wash out air. While flushing, "tap" with a scissor on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and "tapping" must be repeated. When flushing the membrane it may appear to be "sweating" which is due to the ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5µL/min) and check for leaks. The microdialysis probe is now ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation. The probe can be stored in deionized water.
10. For further setup instructions, see CMA 120 System for Freely Moving Animals, User's Manual. 

CMA 12 High Cut-Off Microdialysis Probes

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean, at room temperature, and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Connect the desired length of tubing to the inlet and outlet of the probe. Remove the protection tube carefully. Don't add longer outlet tubing than necessary to avoid ultrafiltration. No longer than 500mm. Short cannula = inlet, long cannula = outlet. Tubing Adapters and FEP tubing should be used for all connections. To facilitate the handling of Tubing Adapters, they should be soaked in Ethanol for minimum 10 minutes. 
4. Attach the Microdialysis probe to a 11 & 12 Probe/Guide Clip on the CMA 130 in vivo Stand. put the probe membrane into a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing.
6. Flush the probe with 10-15µL/min in the Perfusion Fluid for 3-4min to wash out air. When flushing, the membrane may appear to be "sweating" which is due to ultrafiltration of fluid through the membrane. While flushing, "tap" with a scissor on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the air bubble. Lift up the clip with the probe from the vial and check for air bubbles inside the membrane with a microscope. Air bubbles occur as white spots.
7.  Set the pump to the required perfusion flow (usually 1-2µL/min) and check for leaks. Keep pump, probe and tubing at the same level on the bench to prevent ultrafiltration. If the membrane still sweats it might still be air inside the probe. Repeat step 6. It might help to change flow direction in the probe by connect the inlet tubing to the outlet on the probe for a minute. Dextran MW 500kDa 3% can be used to prevent ultrafiltration but be cautious if you are using Swivel as these can be blocked.
8. When the membrane is not sweating the system with the probe is ready for use.
9. During the experiment remember to check the fluid volume in the vials to be as calculated. If a higher flow rate than 1-2µL/min is required it is recommended to use a push-pull system to avoid ultrafiltration.
10. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling times.
11. After the experiment, put the Microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to prevent salt crystal formation.
12. For further set up instructions, see CMA 120 System for Freely Moving Animals, User's Manual.