Designed for dialysis experiments in moving soft tissues such as muscle, heart, skin and adipose tissue, as well as in blood, vitreous fluid of the eye & etc.

• CMA8010435 CMA 20 Elite Probe, 4 mm membrane length, pkg. of 3
• CMA8010436 CMA 20 Elite Probe, 10 mm membrane length, pkg. of 3
• CMA8011430 CMA 20 Elite Probe, 30 mm membrane length, pkg. of 3
• CMA8309670 CMA 20 HighCO Probe, 4 mm membrane length, pkg. of 3
• CMA8309671 CMA 20 HighCO Probe, 10 mm membrane length, pkg. of 3
• CMA8011630 CMA 20 HighCO Probe, 30 mm membrane length, pkg. of 3

CMA 20 Elite Microdialysis Probe for Subcutaneous and Peripheral Tissue Implantation

1. Connect the syringe to the inlet tube by using a tubing connector. To facilitate the handling of Tubing Adaptors, they should be pre-soaked in ethanol for a minimum of 10 minutes. Perfuse the probe with 10-12 µl/min in order to remove air. It is important that the probe is primed with perfusion fluid before insertion in the tissue
2. Anesthetize the rat. Be sure to keep the animal’s body temperature normal during the surgical procedure and the wakening period, which can be achieved by using a homeothermic blanket
3. Place the anesthetized animal on its ventral surface (see fig 2A).
4. Make a small midline incision in the skin to expose the lobular adipose tissue between the scapulae (see fig 2A).
5. If securing with sutures, suture two threads in the muscle, approximately where the wings of the probe will be placed. If securing with cyanoacrylate glue, see step 8.
6. Hold the tissue with forceps while inserting the Introducer with split tubing into the adipose tissue (see fig 1B and 2A).
7. Carefully remove the needle without removing the split tubing. It may be necessary to hold the tubing with forceps (see fig 1C).
8. Insert the probe through the tubing (see fig 1D).
9. Carefully pull upwards and outwards on the split ends of the tubing to tear it apart and remove the tubing (see fig 1F).

Instructions for CMA 20 Elite Microdialysis Probe for Intravenous Implementation

1. Place the anesthetized animal on its back with the tail towards you (see fig 2B).
2. Make a small incision in the skin over the pectoral muscle on one side of the midline (see fig 2B). The large jugular vein is easily seen in young rats, but in older animals it may be concealed by fat. If so, make a blunt dissection to clear the fat and connective tissue. NOTE: Do not attempt to isolate or handle the vein as it will constrict.
3. If securing with sutures, suture two threads in the muscle,  approximately where the wings of the probe will be placed. Take care not to suture too deep into the pectoral muscle. If securing with cyanoacrylate glue, see step 8.
4. Lift the pectoral muscle with toothed forceps and insert the Introducer with the split tubing through the pectoral  muscle into the vein (see fig 1B and 2B). The Introducer should be inserted through the pectoral muscle at an angle of about 10 degrees.
5. Carefully remove the needle without removing the split tubing. It may be necessary to hold the tubing with forceps (see fig 1C). Blood should seep back through the tubing, which indicates that the tubing is in the correct position.
6. Insert the probe through the tubing (see fig 1D). Perform this operation quickly to avoid too much blood loss. NOTE: The pectoral muscle will seal the vein around the probe. The jugular vein does not need to be ligated in order to secure the probe.
7. Secure the probe with sutures on both sides of the wing (see fig 1E). If securing with cyanoacrylate glue, place glue on the underside of the probe wing and secure to the tissue.
8. Carefully pull upwards and outwards on the split ends of the tubing to tear it apart and remove the tubing (see fig 1F). NOTE: Do not tear against the tissue.
9. To protect the probe tubing from damage by the animal, tunnel the inlet and the outlet tubing under the animal’s skin to a point at the skull, nape of the neck or midline of the back. To do so, first make an incision through the skin at the desired exit point. Then insert a cannula of appropriate length and diameter (or hemostatic forceps) through the incision. Push the cannula under the skin to the CMA 20 Elite implant site. Insert the ends of the probe inlet and outlet tubing and gently push them through the cannula (see fig 3). Removing the cannula from the direction it was inserted will exteriorize the probe tubing at the desired site. Secure the exteriorized probe tubing and close both incisions. It is advisable to use some type of secured cover or harness to prevent the animal from damaging the exteriorized tubing.
10. Connect the blue inlet tubing to the CMA 402 or CMA 4004 Microdialysis Pumps (via the swivel). Start the pump on 8-10 µL/min. Observe the transparent outlet tubing to make sure that liquid is flowing. Proceed flushing for approximately 3-5 minutes to remove air bubbles.
11. Before connecting the outlet tubing, reduce the flow rate to operational flow rates, usually 1-5 µL/min.
12. For further set up instructions, see CMA 120 System for Freely Moving Animals, User’s Manual.
13. After the experiment, cut the sutures and remove the probe. NOTE: Once wetted it is recommended that the probe membrane should remain wet.

High Cut Off Probes

Instructions for CMA 20 High Cut-Off Microdialysis Probe for Subcutaneous and Peripheral Tissue Implantation

1. Connect the syringe to the inlet tube by using a tubing connector. To facilitate the handling of Tubing Adaptors , they s hould be pre-s oaked in ethanol for a minimum of 10 minutes . Perfuse the probe with 10-12 µl/min in order to remove air. It is important that the probe is primed with perfusion fluid before insertion in the tissue.
2. Anesthetize the rat. Be sure to keep the animal’s body temperature normal during the surgical procedure and the wakening period, which can be achieved by using a homeothermic blanket.
3. Place the anesthetized animal on its ventral surface (see fig 2A).
4. Make a small midline incision in the skin to expose the lobular adipose tissue between the scapulae (see fig 2A).
5. If securing with sutures, suture two threads in the muscle, approximately where the wings of the probe will be placed. If securing with cyanoacrylate glue, see step 8.
6. Hold the tissue with forceps while inserting the Introducer with split tubing into the adipose tissue (see fig 1B and 2A).
7. Carefully remove the needle without removing the split tubing. It may be necessary to hold the tubing with forceps (see fig 1C).
8. Insert the probe through the tubing (see fig 1D).
9. Carefully pull upwards and outwards on the split ends of the tubing to tear it apart and remove the tubing (see fig 1F).

Instructions for CMA 20 High Cut-Off Microdialysis Probe for Intravenous Implantation

1. Place the anesthetized animal on its back with the tail towards you (see fig 2B).
2. Make a small incision in the skin over the pectoral muscle on one side of the midline (see fig 2B). The large jugular vein is easily seen in young rats, but in older animals it may be concealed by fat. If so, make a blunt dissection to clear the fat and connective tissue. NOTE: Do not attempt to isolate or handle the vein as it will constrict.
3. If securing with sutures, suture two threads in the muscle,  approximately where the wings of the probe will be placed. Take care not to suture too deep into the pectoral muscle. If securing with cyanoacrylate glue, see step 8.
4. Lift the pectoral muscle with toothed forceps and insert the Introducer with the split tubing through the pectoral  muscle into the vein (see fig 1B and 2B). The Introducer should be inserted through the pectoral muscle at an angle of about 10 degrees.
5. Carefully remove the needle without removing the split tubing. It may be necessary to hold the tubing with forceps (see fig 1C). Blood should seep back through the tubing, which indicates that the tubing is in the correct position.
6. Insert the probe through the tubing (see fig 1D). Perform this operation quickly to avoid too much blood loss. NOTE: The pectoral muscle will seal the vein around the probe. The jugular vein does not need to be ligated in order to secure the probe.
7. Secure the probe with sutures on both sides of the wing (see fig 1E). If securing with cyanoacrylate glue, place glue on the underside of the probe wing and secure to the tissue.
8. Carefully pull upwards and outwards on the split ends of the tubing to tear it apart and remove the tubing (see fig 1F). NOTE: Do not tear against the tissue.
9. To protect the probe tubing from damage by the animal, tunnel the inlet and the outlet tubing under the animal’s skin to a point at the skull, nape of the neck or midline of the back. To do so, first make an incision through the skin at the desired exit point. Then insert a cannula of appropriate length and diameter (or hemostatic forceps) through the incision. Push the cannula under the skin to the CMA 20 High Cut-Off  implant site. Insert the ends of the probe inlet and outlet  tubing and gently push them through the cannula (see fig 3). Removing the cannula from the direction it was inserted will exteriorize the probe tubing at the desired site. Secure the exteriorized probe tubing and close both incisions. It is advisable to use some type of secured cover or harness to prevent the animal from damaging the exteriorized tubing.
10. Connect the blue inlet tubing to the CMA 402 or CMA 4004 Microdialysis Pumps (via the swivel). Start the pump on 8-10 µL/min. Observe the transparent outlet tubing to make sure that liquid is flowing. Proceed flushing for approximately 3-5 minutes to remove air bubbles.
11. Before connecting the outlet tubing, reduce the flow rate to operational flow rates, usually 1-5 µL/min.
12. For further set up instructions, see CMA 120 System for Freely Moving Animals, User’s Manual.
13. After the experiment, cut the sutures and remove the probe. NOTE: Once wetted it is recommended that the probe membrane should remain wet.