The Harvard Apparatus-CMA 4004 Syringe pump is an easy to use syringe pump featuring a full color touchscreen, and easy to create configurations. The syringe pump uses a microstepping motor to  reduce flow pulsation. The syringe pump accuracy is 0.35% and has a wide dynamic flow rate range from 0.54pl/min to 11.70ml/min (dependent on syringe size). 

CMA 4004 Syringe Pump Series Touch Screen Button Reference

Operating Instructions

1. Plug the power cord into the power source on the back of the pump. The CMA 4004 Syringe Pump Series has a DC power input connector on the rear of the unit. The operating range for the CMA 4004 Syringe Pump Series is 12-30VDC.
2. Turn on the main power switch.

Syringe Loading

The CMA 4004 Syringe Pump Series can accommodate up to 4 syringes. Any type of syringe, glass, plastic or stainless steel can be used. The CMA 4004 Syringe Pump can accommodate a single syringe from 0.5µl to 10ml.

1. Press the release button (1) and move the pusher block (2) to the approximate length of the syringe.
2. Unscrew the bracket clamping knobs (7,8) to loosen the retaining brackets (4,5) on the pusher block (2) and the Syringe holder block (3).
3. Unscrew the syringe barrel clamp by unscrewing the syringe barrel clamp knob (6).
4. Place the syringe on the syringe holder block (3). Ensure the barrel flange is with in the syringe holder block's retaining bracket (5) and the plunger flange is in the pusher block's retaining bracket (4).
5. Screw down the syringe clamp knob (6) until the syringe barrel clamp (10) is tight against the syringe barrel. Do not overtighten, especially when using glass syringes.
6. Secure the syringe flanges by pushing the retaining brackets (4, 5) tightly against the syringe flanges using the bracket clamping knobs (7, 8). The syringe plunger should be secured by the pusher block retaining bracket and the flange of the syringe should be secured by the syringe holder block retaining bracket.
7. Tighten the mechanical stop collars (9) using Allen wrench (11) to prevent excess travel of the pusher block.

Configuring the CMA 4004 Syringe Pump Series

Setting up a Method

Method Selection

The Method Selection Screen will be display. The available Methods will be displayed based on the model of syringe pump.

Press the Method to Select

Syringe Selection

In the Quick Start Screen select the syringe button.

Select the manufacturer type. Then the available syringe sizes will be displayed. In this example we have selected a Becton-Dickinson Syringe.

For a Custom Syringe use this selection if syringe manufacturer is not listed.

Custom Syringe Definition Display

Flow Rate Selection

In the Quick Start Screen enter the flow rate(s). In this example we want to enter 75 ml/min.

Depending on the Method, the appropriate set rate screen will be displayed. To change the units, press the units button. 

In the Units Selection Screen select the volume and the time base.

Target Volume/Time Selection

In the Quick Start Display enter the target volume or time. In this example we want a volume of 60ml.

Depending on the Method either Target Volume (I/W or W/I) will appear or Target Volume or Target Time Selection (I only or W only).

To change units, press the units. 

In the Units Selection Screen select the volume units.

Previewing the Quick Start

It is simple to view the Pump Quick Start on the RUN display.

Press to view Pump Quick Start

After you have verified the configuration. Press to run the displayed configuration.

Configurating the Pump's General Parameters

The pump has basic parameters that should be configured during initial start-up. Once these parameters are entered they probably won't be changed. 

To enter the setting screen press

Setting the Audible Alarms

In the settings screen, press Audible Alarms.

Select the alarms, a checkmark will indicate the alarms selected.

If these alarms are selected the pump will provide an audible alarm.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

End of Run Warning

To set an alarm warning of the end of run. Select the % volume dispensed. For sample, if you select 80% the pump will sound an alarm (3 short beeps) when 80% of the volume is dispensed.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

For a Custom %

If you would like to use a custom percentage for an end of run warning, use "Set Other Value."

Enter the percentage.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Locking the Touchscreen

A touchscreen lock function can be enabled to prevent accidental operation or interruption of operation. Unlocking the touchscreen requires a password, defined by the user. 

Setting the Password

To set the Password go to the Settings screen. Press the Set Password button. 

Enter your desired password, up to 8 digits, and press the "ACCEPT" button.

Turning the Touchscreen Lock On

Press the Lock button twice to lock the touchscreen.

Turning the Touchscreen Lock Off

Press the "LOCK" button to bring up the Enter and Pump Password screen.

Enter your password and press the "ACCEPT" button.

Setting the Force Limits

The maximum linear force of the syringe pump is 35lbs or 16kgs. This force is sufficient to damage delicate, low volume syringes, as well as many standard syringes if pumping viscous fluids. The % of the maximum force applied by the pusher block can be changed.

In the settings screen, press Force.

Set the Force Level.

Select the Force Level %.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

For a Custom Force %

Press Set Other Value to enter a custom force %

Enter the percentage

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Note: A minimum value of approximately 20% is required to overcome friction forces in the mechanism. You may have to set the force level to a higher value depending on the back pressure/friction in your system.

Setting the Display Backlight

The backlight can be adjusted. In the Settings screen, select Backlight.

Select the % Backlight. For example, if you select 80%, the brightness of the display will be 80% of the maximum.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

For a custom value.

Use this selection if the % is not listed. 

Press Set Other Value to enter a custom %

Enter the percentage.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Setting the Pump Address

Up to 100 pumps can be linked together and each pump must be given a unique address.

In the Setting Screen press Pump Address. Valid entries are 00 through 99.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Setting the Baud Rate

The CMA 4004 Syringe Pump Series has an RJ-11 option for serial communication.

To adjust the baud rate from 9600 to 115,200 in the Settings screen press Baud Rate

Select the Baud Rate

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Power-Up Running

If Power-Up Running is selected the pump will resume running when power is restored to the unit. This is most often used if there is a concern about loss of power during a run.

In the Setting screen, select Power-Up Running

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Note: This features is only valid in Infuse Only or Withdraw Only Methods. This feature will only execute if the pump is running at the time when the power is lost, and was running with no target set.

Note: Elapsed timers and total infused volume will be reset when power is restored. Counters and timers information prior to loss of power are lost. Pump will stop at the position of the stop collars. 

Setting and Viewing Pump Information

The information on the pump parameters are displayed in the Pump Information Screen.

In the Settings screen select pump information.

The current information on the pump will be displayed.

• Model Type
• Software Version
• Maintenance Due
• Current Pump Address

Press Maintenance Reset to reset the # of hours for maintenance. This will reset it to 100hrs.

Confirm the maintenance reset by pressing the Maintenance Reset again.

Accept the changes made by pressing and go back to the settings screen.

Cancel the changes made by pressing and go back to the settings screen.

Operating the Pump

The CMA 4004 Syringe Pump is easy to operate, due to the easy to see parameters on the RUN screen. Depending on the Method of the pump, different parameters will be shown on the display.

Running the Pump

Simply press the button to start the pump.

When the pump is running the button is shown.

Press the button to pause the method.

Press the to resume running the pump.

Press the to clear the counters and timers. 

The fast forward and fast reverse keys are not active when the pump is running.

Custom Method Workflow

The following flow diagram describes the use of a custom method:

The software provides the user with a variety of prompts and warnings to help guide the setup of Methods. For example, if a pump Method incorporates steps that exceed the capacity of the defined syringe, the system will prompt you that parameters are out of range. The system also prompts users if required values are not entered.

NOTE: The pump will check the validity of parameters entered for each step in the method, but will not review the method in its entirety to verify that it is logical and can be fully executed.

Note: See page 30 of the user manual for a step-by-step operation example using a custom method.

Configuring a New Custom Method

To display the Method Selection screen press Method.

The Method Selection screen will be displayed

To go up a page in methods use

To go down a page in methods use

Configuring Custom Methods

The first thing to do on a custom method is to name the method.

Enter any 15 character alphanumeric name.

Accept the new name

Go back to the Method Selection Screen

Select the syringe manufacturing and size.

To define the steps for the custom method press Step Definition.

Below are some predefined steps including:

• Constant Rate - a single continuous rate of flow
• Ramped Rate - a linearly changing flow rate between a starting and ending flow rate
• Autofill - continuous infuse/withdraw or withdraw/infuse operation
• Gradient - combined flow streams from multiple pumps into a common output stream

In addition to these you can repeat steps or set a delay within a Method as well as send signals to external devices or react to signals received from external devices. This package of pumping profiles and commands provides users with an extremely broad array of applications that can be easily configured with complex external programming.

Methods can be edited and saved under a new name, allowing them to be simply edited to fit another application. Steps can be copied, moved, inserted or deleted. 

Setting a Delay

You can set the CMA 4004 Syringe Pump Series to run after a delay. This delayed run function allows you to set up all the parameters for operation, then have the pump start unattended after a specified amount of time.

Setting a delay for method operation

From the Quick Start or Method Main screen, choose the Set Delay Parameters button to enter the Set Delay.

Enter the desired Time using the keypad.

If the Units of your Tim are not correct for your application, select the Units button to toggle between seconds (sec) and hh:mm:ss option, enter your time value in hours, minutes, and seconds, separating each value with a colon. You can enter a value up to 99:99:99.

When the desired values are entered, choose the Accept Button to save your Changes and return to the Method Quick Start screen. When a Time Delay value is active, the Time Delay Parameters button is diplayed in Red on the screen to alert the user that a delay has been set.

Press the Run button to activate the Delay. 

Creating a New Custom Method

If none of the Methods stored on the CMA 4004 Syringe Pump Series match your requirements, you can create a new Method and store the Method for use. The following flow diagram outlines the basic steps you will follow to create a new Method.

To create a new user-defined method

From the Method Quick Start or Method Main screen, choose the Method Select button to enter the Method Selection screen.

Select New Method then choose Accept or press New Method again.

On the Method Name screen, type the name of your method using the onscreen keyboard. Choose the Symbols/Numbers Button to display the list of available numbers and symbols that can be included in the Method Name. To switch the keyboard back to letters Method, choose the Letters button (ABC).

When you have entered the desired name for your Method, choose the Accept button to go to the Method Main screen. Your new Method will now be listed in the Method Select button.

Choose the Syringe Select button and complete the description of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide.

To begin creating Pump Control Profile Steps, choose the Step Definition button to enter the Method Steps Summary screen.

To add a Step to your Method, choose the Add Step button to access the Method Steps Menu screen. The available options include:

Choose the Method Step type that you want to add to the Method. if you choose a pumping profile (e.g. Constant Rate, Ramp, etc.) you will be taken to the Step Definition screen for the selected profile.

Complete the parameter entry applicable to the selected Pump Operation Profile, follow the detailed instructions supplied in the Pump Operation Profiles (Existing and User-Defined Methods) section later in this Guide.

NOTE: If you do not complete the required parameter entry for a Step, subsequent Step additions may be ignored. You should enter all required parameters for a Step when creating it to ensure that the application software can assemble your Method without errors.

When you have completed all parameter entries on the Step Definition screen, choose the button to save your changes and return to the Method Steps Summary screen.

Repeat items 7-10 in this procedure to include additional Steps in the Method.

NOTE: When adding a Step to a Method, the Step is added after the currently selected Step (i.e. the Step that is highlighted in magenta)

When you are done adding all required Steps to the Method, choose the button to return to the Method Main Screen.

Modifying an Existing User-Defined Method.

Once you have created a Method or have reviewed an Existing Method, you may have a need to modify the structure of the Method (i.e. the types of Steps and Commands included, not just changes in the operating parameters for a specific Step. The following procedures will help you to efficiently modify a stored Method.

Changing the order of steps in a method.

From the Method Main screen, choose the Step Definition button to enter the Method Steps Summary screen.

Highlight (select with a single touch) the Step for which you want to change the order.

Choose the Move Step Up or Move Step Down button, as required, to move the Step into the position that you want it executed. 

Deleting a step from a method.

From the Method Main screen, choose the Step Definition button to enter the Method Steps Summary screen. 

Highlight (select with a single touch) the Step that you want to delete.

Choose the Delete Step button. The application will display a warning message in the Message Area asking you to confirm your deletion request.

To confirm the deletion, choose the Delete Step button again. 

Defining Method Step Parameters

This section of the User Guide describes each of the functions available for use in the CMA 4004 Syringe Pump Series Existing and User-Defined Methods. The information in this section is designed to help you set the individual parameter values in each of the Method Step options and to properly use the Advanced Options commands.

The information that follows is organized into two sections:

• Pump Operation Profiles
• Advanced Options (Commands and I/O)

Pump Operation Profiles

Constant Rate Profile

The constant rate profile is used to run the pump at a defined flow rate and for a specific pumping mode (infuse or withdraw) up a target volume or for a defined time period.

When creating or editing a Constant Rate profile, you will define three primary operating parameters:

• The Flow Method defining the direction of flow: infuse or withdraw
• The Flow Rate to define the rate or uptake or dispensing
• The Target Volume or Run Time that determine how long the pump will operate at the set rate.

When creating or editing a Constant Rate profile, you can set the rate in ml, µl, nl or pl units of volume using sec, min or hr as units of time. The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom-defined. The min/max values are made available as button selection options on the Set Infuse and Withdraw Rate screens.

To set-up/edit a constant rate profile:

• From the Step Definition screen for a constant rate profile, choose the set mode button to toggle the flow method between infuse and withdraw operation.
• Choose the set rate button to enter the set rate screen.
• Enter the flow rate value desired using the keypad or select the minimum rate or maximum rate button.
• If the units of rate are not correct for your application choose the units button.
• On the units selection screen select the unit of measure (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose the units button.
• When all parameters are correction set on the set rate screen choose to confirm your settings and return to constant rate definition screen. 
• Choose the Set Target Volume or Time button to enter the set volume or time screen.
• On the set volume or time screen select the volume or time button as desired.
• Enter the desired value using the keypad or select the maximum volume button.
• If the units of volume/time are not correction for your application choose the "Units" button to the right of the parameter entry box. For the "Units of Volume" this choice takes you to the units selection screen. For the "Units of Time" option this choice will toggle the button between seconds (sec) and hours:minutes:seconds (hh:mm:ss). To use the hh:mm:ss option, enter your time value in hours, minutes and seconds separating each value with a colon.
• If you are changing the "Units of Volume" on the "Units Selection" screen, select the unit of measure (ml, µl, nl or pl) as appropriate, then choose to confirm your selection.
• When all parameters are correctly set on the set "Target Volume" or "Time Screen" choose to confirm your settings and return to the "Constant Rate" definition screen.
• Choose again to return to the "Step Definition" screen.

Ramp Profile

The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping method (infuse or withdraw) over the course of a defined time period.

When creating or editing a Ramp Rate profile, you will define three primary operating parameters:

• The Flow Method defining the direction of flow: infuse or withdraw
• The Flow Rate to define the rate or uptake or dispensing
• The Target Volume or Run Time that determine how long the pump will operate at the set rate.

During a Ramp Rate profile the CMA 4004 syringe pump varies the flow rate linearly between the starting rate and ending rate over the full time period entered. A Ramp Rate profile can be applied to an increasing or decreasing rate application.

When creating or editing a Ramp Rate profile you can set the rate in ml, µl, nl or pl units of volume using sec, min or hr as units of time. The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom-defined. The min/max values are made available as button selection options on the Set Infuse and Withdraw Rate screens.

To set-up/edit a ramp rate profile:

• From the Step Definition screen for a constant rate profile, choose the set mode button to toggle the flow method between infuse and withdraw operation.
• Choose the "Set Start" and "End Rate" button to enter the "Set Start" & "End Rates" screen.

• Select the "Set Start" and "End Rate" button (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the flow rate value desired using the keypad or select the "minimum rate" or "maximum rate" button.
• If the units of rate are not correct for your application, choose the units button.
• On the units selection screen select the unit of measure (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose to confirm your selections.
• Repeat steps 3 through 5 for the end rate by selecting the end rate button and continuing data entry.
• When all parameters are correctly set on the set rate screen and choose to confirm your setting and return to the Ramp Definition screen.
• Choose the "time" button to enter the set time screen.
• Enter the desired time value using the keypad.
• If the units of time are not correct for your application select the "units" button to toggle between seconds (sec), minutes (min) and hours (hr). To use the h:m:s option, enter your time value in hours, minutes and seconds, separating each value with a colon.
• When all parameters are correctly set on the "Set Target Time" screen, choose to confirm your settings and return to the "Ramp Definition" screen.
• Choose again to return to the "Step Definition" screen.

NOTE: Ramps "2 seconds from min to max rates may cause motor stalls.

Gradient Profile

The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream. The flow profiles can be constant, ramped or stepped.

• When creating or editing a gradient profile you will define 4 primary operating parameters:
• The combined flow rate.
• The target starting and ending percentages for each pump in the system.
• The target time that determines the total length of time over which the gradient profile will be calculated.
• The number of steps that will be created in that profile. 

NOTE: See Appendix C: RS-485 for physical gradient connections in the attached manual.

NOTE: Control pump address must be set to 00. Ensure that any satellites' address matches the address set in the gradient step.

CAUTION: All pumps must use the same syringe size and type. If there is a syringe change on the control unit then the same change must occur with any satellite or remote pump.

NOTE: Make all cable connections, then power-up the satellite or remote pump before powering up the control pump. 

To set-up/edit a Gradient Profile:

• From the step definition screen for "Gradient" choose "Set Combined Flow Rate" to enter the rate screen.

• Enter the desired combined flow rate value for both pumps using the keypad or select the "Minimum rate" or "Maximum Rate" button.

• If the units of rate are not correct for your application, choose the units button.
• On the units selection screen select the unit of measure (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose to confirm your selections.
• When all parameters are correctly set on the set rate screen, choose to confirm your settings and return to the step definition screen.
• Choose the "Set Percentage" button for pump 00.
• Set the Start and End Percentages for pump 00 then choose .
• Choose the "Address" button, and then set the address of the second pump. Choose to return to the step definition screen. 

• The pump will calculate the remaining percentages for the second pump.
• Choose the "Set Time and # Steps" button. Enter the total amount of time and the number of steps desired and then choose .

NOTE: For a constant rate or ramp profile using a gradient method choose 0 steps. For a stepped profile using a gradient method select the number of steps desired.

NOTE: When running a gradient step, ensure that no unused pumps are included in the pump chain. 

Running a Gradient Profile:

The pump control button's background color indicates the status of the satellite unit.

Blue Background = Connected / Motor Stopped

Red Background = Comm Error

Yellow Background = Motor Stall

Green Background = Motor Running

NOTE: When running a gradient step, the satellite pump's screen will display "Satellite" followed by its pump address. A stop button will be active on the satellite when the pump is running. 

Autofill Cycling Profile

The Autofill Cycling Profile is an automated Infuse/Withdraw or Withdraw/Infuse step that operates at constant, but separately defined, flow rates for the withdraw and infuse operations. The Autofill profile is designed to work in conjunction with an autofill valve that controls the switching of flows from a reservoir for refilling the syringe, to the target infusion subject.

When creating or editing an Autofill Cycling profile, you will define the following operating parameters:

• The flow mode defining the cycle: Infuse/Withdraw (INF/WD) or Withdraw/Infuse (WD/INF).
• The flow rates to be used during Infuse/Withdraw operations.
• The infusion volume to be delivered during each pump cycle.
• The total volume to be delivered during all cycles or the total number of Infuse/Withdraw or Withdraw/Infuse cycles that you want the pump to execute. For example, if you want to pump 100 ml using a 10 ml syringe, the pump will execute 10 Infuse/Withdraw or Withdraw/Infuse cycles, depending upon the chosen flow mode.

During an Autofill profile, the CMA 4004 Syringe Pump operates at a constant pump rate during the infusion and withdrawal stages, although the rate can be different for each direction (i.e. infuse vs withdraw).

When creating or editing an Autofill Cycling profile, you can set the rate in ml, µl, nl or pl units of volume using sec, min or hr as units of time. The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom-defined. The min/max values are made available as button selection options on the Set Rate and Concentration screen.

To Set-Up/Edit an Autofill Cycling Profile:

• From the Step Definition screen for an Autofill Cycle, choose the "Set Mode" button to toggle between the Withdraw/Infuse (WD/INF) and Infuse/Withdraw (INF/WD) modes. Withdraw/Infuse begins by withdrawing into the syringe, following by infusing into the target.
• Choose the "Set Rates" button to enter the "Set Infuse/Withdraw Rate" screen.
• Select the Infuse Rate (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button.
• If the "Units of Rate" are not correct for your application, choose the "units" button.
• On the "Units Selection" screen, select the unit of (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose to confirm your settings and return to "Step Definition" screen.
• Choose the "Set Volume per Cycle" button to enter the "Set Volume per Cycle" screen.
• Enter the Volume using the keypad.
• If the units of time are not correct for your application select the "units" button to toggle between seconds (sec), minutes (min) and hours (hr). To use the h:m:s option, enter your time value in hours, minutes and seconds, separating each value with a colon.
• When all parameters on the "Set Volume per Cycle" screen are correct, choose the button to return to the "Step Definition" screen.
• Choose the "Set Total Volume or # Cycles" button to enter the "Set Total Volume or # Cycles" screen.
• Select either "Total Volume or # Cycles" to define how the pump will control pumping activity. Total Volume will cycle the pump the correct number of times to deliver the total volume according to the "Volume per Cycle" setting. For example, if you set a 10 ml Volume per Cycle and set 100 ml in Total Volume, the pump will cycle 10 times.
• If you select "Total Volume" enter the volume desired using the keypad. If you select # cycles, enter the total number of Infuse/Withdraw or Withdraw/Infuse cycles that you wish to perform.
• If the units of measure associated with volume are not correct for your application, choose the "units" button to enter the units selection screen. Select the unit of measure button (ml, µl, nl or pl) appropriate for your application, then choose the button to return to the "Set Target Volumes" screen.
• When all parameters on the "Set Total Volume or # Cycles" screen are correct, choose the button to return to the Step Definition Screen.

NOTE: Using maximum rate may not be appropriate during the Withdraw stage of an Autofill profile, since air can be drawn into the syringe, or a high vacuum pressure can be produced depending on the syringe and/or tubing being used, or the fluid viscosity. 

Concentration Profile

The Concentration Delivery profile is used to manage infusion of a defined concentration of drug or other liquid to set dosage level based on the subject's weight.

When creating or editing a "Concentration Delivery" profile, you will define the following operating parameters:

• The weight of the target subject.
• The flow rate to be used during infusion.
• The concentration of the drug or other liquid being infused.
• Either the target dosage or the target infusion time -- the pump automatically calculates whichever value you do not enter. 
• If you wish to infuse multiple doses to the target, you can specify the number of doses along with a delay between dosings.

During a Concentration Profile, the CMA 4004 operates at a constant infusion rate and automatically stops when the target dosage rate is reached.

When creating or editing a concentration flow profile, you can set the rate in ml, µl, nl or pl units of volume using sec, min, or hr as the units of time. The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom-defined. The min/max values are made available as button selection options on the "Set Rate" and "Concentration" screen. The ingredient concentration is entered in mg/ml.

To set-up/edit a concentration delivery profile:

• From the Step Definition screen for a concentration profile, choose the "Animal Weight" button to enter the "Set Animal Weight" screen.

• Enter the Weight in grams then choose the button to return to the step definition screen.
• Choose the "Set Rate and Concentration" button to enter the "Set Rate and Concentration" screen.

• Select the infuse rate (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the desired rate using the keypad or select the "Min Rate" or "Max Rate" button.
• If the Units of Rate are not correct for your application choose the units button.
• On the units selection screen, select the unit of measure (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose to confirm your selections.
• Select the "Concentration" button and enter the concentration of the active ingredient in the infusion fluid in mg/ml.
• When all parameters are correctly set on the "Set Rate and Concentration" screen, choose to confirm your settings and return to the "Step Definition" screen.
• Choose the "Set Dose or Duration" button to enter the "Set Dose or Duration" screen.
• On the "Set Dose or Duration" screen, select either the Dose button (if you wish to enter the target dosage value in which case the CMA 4004 will calculate the required pumping duration) or Duration (if you wish to infuse for a specific time period in which case the CMA 4004 will calculate the delivered dose).
• As appropriate enter either the Dose in mg/ml or duration using the keypad.
• If the units of time associated with duration are not correct for your application, select the units button to toggle between seconds (sec) and hours:minutes:seconds (h:m:s). To use the h:m:s option, enter your time value in hours, minutes and seconds, separating each value with a colon. 
• When all parameters on the "Set Dose" or "Duration" screen are correct, choose the button to return to the Step Definition screen.
• Choose the Set # Doses & Time Between Doses button to enter the Set # Doses & Time Lag screen.

• Select the "Does" button (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the desired number of doses using the keypad.
• If the # Doses is greater than one, select the "Time Lag" button and enter the desired length of time between individual doses.
• If the Units of Time associated with Time Lag are not correct for your application, select the units button to toggle between seconds (sec), and hours:minutes:seconds (h:ms). To use the h:m:s option, enter your time value in hours, minutes and seconds, separating each value with a colon.
• When all parameters on the "Set # Doses & Time Lag" screen are correct, choose the button to return to the step definition screen.

Incorporating Advanced Options (Commands and I/O) into a Method.

CMA 4004 Syringe Pump Series Methods can incorporate a variety of commands that expand the versatility of the system by permitting convenient looping, chaining of events, sending signals to external devices, or accepting inbound signals from external devices. These Advanced Options controls are described in this section of the Guide.

Delay

The delay command is used to insert a time delay in a procedure. You can set a time delay between 0.2seconds up to 99:99:99 (99 hours, 99 minutes, 99 seconds).

To Insert a Time Delay in a Method:

1. From the Method Main screen, choose "step definition" to enter the "step definition" screen.
2. Highlight (select once) the Method Step that you want the time delay to follow.
3. Choose the Insert Step button to access the Method Steps Menu screen.

4. Choose Delay to enter the Set Delay Time screen.

   

5. Enter the desired time using the keypad.
6. If the Units of Time associated with time are not correct for your application, select the "Units" button to toggle between seconds (sec) with 0.2 seconds the minimum allowable value and hours:minutes:seconds (h:m:s). To use the h:m:s option, enter your time value in hours, minutes and seconds, separating each value with a colon. You can enter a value up to 99:99:99
7. When your time entry is correct, choose the button to return to the step definition screen.

Repeat Step

The Repeat Command is used to repeat steps in a procedure a defined number of times. To use this command, you will tell the application what steps you want to repeat and how many times they should be repeated. The repeat step command creates a "ump" back to a specific step in a method that executes the defined step plus all following steps up to the repeat command (creating a method loop). 

When defining a repeat jump in a method, the command targets the step sequence position and not the function that currently occupies that position. For example, if you create a repeat jump to "Step 4" in a method (currently a ramp pump operation profile) into the "Step 4" position, the repeat jump will then connect to the constant rate operation and not to the ramp operation.

NOTE: DO NOT USE NESTED LOOPS 

To Insert a Step Repeat Sequence in a Method:

1. From the method main screen, choose step definition to enter the step definition screen.
2. Highlight (select once) the method step that you want the repeat step to follow.
3. Choose the button to access the method steps menu screen.
4. Choose "Repeat" to insert the repeat step function into the method. 
5. Select the step(s) you would like to repeat from by selecting the step button twice or selecting once and then choosing .

6. 

7. Enter the number of repeats using the keypad then choose the button to save your change and return to the step definition screen.

NOTE: This command creates a loopback to the designated step in a method, executing the selected step plus all subsequent steps up to the point of insertion of the repeat command. For example, if the repeat command is set to repeat step 2 one time, the system will loop back to step 2 and execute both steps 2 and 3 one additional time. 

Stop